Basic methods in protein purification and analysis :a laboratory manual edited by Richard J. Simpson, Peter D. Adams, and Erica A. Golemis.
Material type:
TextPublication details: Cold Spring Harbor, N.Y. : Cold Spring Harbor Laboratory Press, 2009Description: vii, 436 p. : ill. ; 29 cmISBN: - 9780879698676 (pb)
- Proteins -- Purification -- Laboratory manuals
- Proteins -- Analysis -- Laboratory manuals
- Proteins -- isolation & purification -- Laboratory Manuals
- Chromatography -- methods -- Laboratory Manuals
- Electrophoresis -- methods -- Laboratory Manuals
- Immunoprecipitation -- methods -- Laboratory Manuals
- Proteins -- analysis -- Laboratory Manuals
- 572.6078 SIM/Bas 22
- QP551 .B265 2009
- 2008 M-826
- QU 25
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Goa University Library DBT (Biotechnology dept.) | 572.6078 SIM/Bas (Browse shelf(Opens below)) | Available | 163819 | ||
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| 572.6 WRI/Fun Fundamentals of Proteomics | 572.6 WYA/Pro Proteomics :Principles, techniques and analysis | 572.6078 NED-NEL New and Emerging Proteomic Techniques | 572.6078 SIM/Bas Basic methods in protein purification and analysis :a laboratory manual | 572.62 SCO/Tec Technologies, Strategies and Applications of plant Proteomics | 572.633 BAI-NUS Protein Folding Protocols | 572.633 XU/Com -2 Computational Methods for Protein Structure Prediction and Modeling Volume 2 Structure Prediction |
Includes bibliographical references and index.
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized pH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools : analysis for creating focused interaction networks.
Introduction to protein purification strategies -- Preparation of cellular and subcellular extracts -- One-dimensional polyacrylamide gel electrophoresis -- Preparative two-dimensional gel electrophoresis with immobilized PH gradients -- Ion-exchange chromatography -- Size-exclusion chromatography -- Reversed-phase high-performance liquid chromatography -- Affinity and immunoaffinity chromatography -- Metal chelate affinity chromatography -- Multidimensional chromatography of intact proteins -- Identification of protein-protein interactions by conventional column chromatography -- Identification of associated proteins by coimmunoprecipitation -- Chromatin immunoprecipitation of protein complexes -- Identification of protein-protein interactions with glutathione-s-transferase fusion proteins -- Direct visualization of protein interactions in living cells using bimolecular fluorescence complementation analysis -- In silico tools to launch protein interaction analysis : creating focused networks around proteins of interest.
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